Use of Intraductal Adenovirus Transduction to Assess the Mammary Tumorigenic Potential of a Constitutively Active Prolactin Receptor
Abstract
We have constructed adenovirus including PRLR and DELTA PRLR (the constitutively active form), AKT, kinase dead AKT, the oncogenes fyn and src and DELTA AKT (the activated form). In addition a number of control vectors containing green fluorescent protein and Beta-galactosidase are available. Other constructs relevant to the mammary gland in addition to those listed in the proposal including progesterone receptor (PRA and PRB) have been made. We have found that adenovirus transduction of the mammary epithelium persists up to 3 weeks in vivo and that we obtain transduction of about 20% of the cells. Green fluorescent protein can be visualized in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA382973
Entities
People
- Margaret Neville
Organizations
- University of Colorado Boulder