Treatment of Breast Cancer with Immunogenic Cells Transfected with DNA from Breast Cancer Cells
Abstract
This investigation was based on the hypothesis that weakly immunogenic, breast cancer- associated antigens, the products of mutant or dysregulated genes in the malignant cells, will be expressed in a highly immunogenic form by a semiallogeneic IL-2 secreting fibroblast transfected with DNA from breast cancer cells. To investigate this question, we transfected LM mouse fibroblasts (H2k) modified to secrete IL-2 with genomic DNA from breast adenocarcinoma that arose spontaneously in a C3H/He mouse (H2k) . To increase their nonspecific immunogenic properties, the fibroblasts were also modified before transfection to express allogeneic MHC determinants (H-2Kb) . Afterward, the IL-2 secreting semiallogeneic cells were cotransfected with DNA from the spontaneous breast neoplasm, along with with a plasmid (pHyg) conferring resistance to hygromycin. Pooled colonies of hygromycin resistant cells were then tested in C3H/HeJ mice for their immunogenic properties against the growth of the breast neoplasm. The results indicated that the tumor-bearing mice immunized with the transfected cells survived significantly longer than mice in various control groups.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2000
- Accession Number
- ADA383055
Entities
People
- Dubravcka Markovic
- Edward Cohen
Organizations
- University of Illinois at Chicago