Tumor Suppressor Genes in Early Breast Cancer and its Progression
Abstract
Ductal carcinoma in situ (DCIS) is a pre-invasive stage of breast cancer in which the malignant cells have not penetrated the basement membrane. From our allelotyping study of DCIS, chromosomal regions of 8p, 13q, l6q, 17p and 17q showed loss of heterozygosity (LOR) significantly above background (5%). In our previous report we described the LOR mapping of a region on 8p (30% LOR) containing a putative tumor suppressor gene (TSG) which we refined to a small interval of 1.4 cM between the markers D8S265 to D8SD520. Last year (1998) we completed the construction of a fine structure radiation hybrid map and an integrated YAC/BAC contig spanning the 1.1 -cM deletion region. During the past project year, we mapped eleven expressed sequence tags (ESTs) including 4 previously reported ESTs to this contig. Twenty-three overlapping oligo (overgo) probes were generated using end sequences derived from CITB BAC clones. These overgos were used to screen RPCI-l 1 BAC library. A total of 299 clones including CITB BAC clones were isolated and then fingerprinted. A comprehensive sequence-ready contig that provides a useful resource for sequencing the critical region has been assembled using clones with good fingerprinting data.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA383203
Entities
People
- Alison M. Goate
Organizations
- University of Washington