Genetic Regulation of Lipid Biogenesis in Human Breast Cancer

Abstract

It has been shown that dietary n-6 polyunsaturated fatty acids (PUFAs) promote tumorigenesis and maintain growth of breast cancer cell lines, while n-3 PUFAs generally reduce the incidence and development of cancer and inhibit growth in cell culture. We investigated the effects of various n-6 and n-3 PUFAs, on lipogenic gene expression in two breast cancer cell lines, MDA-MB-231 a p53-/- cell line and MCF-7 a p53+/+ cell line. In both lines, we showed that PUFAs repressed the expression of two important genes in the fatty acid biosynthesis pathway, fatty acid synthase (FAS) and stearoyl-CoA desaturase-1 (SCD-1). In addition we showed that this PUFA-induced repression was mediated through the sterol regulatory element binding protein 1 a (SREBP-1a) and not the mutant p53 protein as we had previously hypothesized. SREBP is a newly identified and critical central activator of both the cholesterol and fatty acid biosynthesis pathways.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA383396

Entities

People

  • James Ntambi

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Albumins
  • Anabolism
  • Breast Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemistry
  • Cultured Cells
  • Fatty Acids
  • Gene Expression
  • Lipids
  • Mrna
  • Neoplasms
  • Proteins
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology