Applications of a Novel Nucleic Acid Detection Method in Breast Cancer Analysis of Overexpression of HER-2/neu and FAK

Abstract

The routine, low-cost detection of genetic material is an important step in translating the advances in cancer genetics to therapeutics and diagnosis. In this study, metal oxide electrodes and a solution oxidant of DNA have been used to detect electrochemically the products of the polymerase chain reaction (PCR) performed on niRNA from the HER-2 gene in BT-474 cancer cells. This method can be used to detect as little as 500 attomoles of DNA quickly and at low cost. The technique has recently been expanded to include detection of the size of PCR fragments, which is reflected in the size of the measured currents. This technique has been applied to sequences that are particularly difficult to size and amplify, such as trinucleotide repeats.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA383946

Entities

People

  • Herbert Thorp

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Acids
  • Analytical Chemistry
  • Breast Cancer
  • Catalysis
  • Chain Reactions
  • Chemical Compounds
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Electrodes
  • Molecules
  • Nucleic Acids
  • Organic Chemistry
  • Oxidation
  • Oxides
  • Polymerase Chain Reaction

Fields of Study

  • Chemistry

Readers

  • Image Processing and Computer Vision.
  • Molecular Genetics
  • Oncology

Technology Areas

  • Biotechnology