Analysis of a Novel 17q25 Cell Cycle Gene Homolog: Is it a Breast Tumor Suppressor Gene?
Abstract
While a subset of the growth regulating genes involved in breast tumorigenesis has been identified, a complete understanding of all culprit genes is essential for the effective translation of these molecular reagents into successful tools for the medical management of breast cancer. We hypothesize that a 350 kb region on 17q25 detected by our allelic imbalance studies harbors a novel breast tumor suppressor gene. The purpose of this investigation is to characterize the role of this novel gene in normal and tumor development. In the past year, 10 cDNA fragments showing unique expression patterns on multitissue Northern blots have been identified. Extensive characterization of one septin GTPase revealed altered bands on both Southern and Northern blots of breast tumor cell lines. This gene was simultaneously identified as a fusion partner with MLL in a leukemia patient and named MLL septin-like fusion (MSF). Although no functionally significant nucleotide alterations have been found in the MSF coding region to date, MSF remains a strong candidate for the breast tumor suppressor gene, and further expression and functional analyses are underway. As well, PCR-based and hybridization-based methods are being used to isolate complete transcripts from other cDNA fragments in the region.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA384018
Entities
People
- Linda Kalikin
Organizations
- University of Michigan