Complementation Screening in Mammalian Cells: Application to Cell Immortalization
Abstract
The broad goals of the work supported by this grant are twofold. The first is to develop and deploy a facile system for complementation screening in cultured mammalian cells. The second is to understand elements of cellular mortality control. With regard to the first, we have developed and deployed the MaRX system. MaRX is a retroviral vector that is specifically designed to facilitate the delivery and recovery of complex gene libraries. Thus far, we have used this system to identify genes that regulate telomerase, to elucidate potential mechanisms of TGF-b-resistance in human breast tumors, to isolate genes that confer resistance to oncogene- induced apoptosis, to find cDNAs that allow bypass of p53-induced growth arrest and to address the reversibility of immortalization processes in murine and human cells. With regard to the second broad goal, we have demonstrated that an oncogene, c-myc, can regulate telomerase activity in normal human cells Efforts over the past year have been aimed at understanding the relevance of telomerase in the immortalization of multiple cell types, at understanding the relationship between myc and telomerase activity in multiple cell types and at developing model systems in which the role of myc in cellular transformation can be addressed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA384034
Entities
People
- Gregory Hannon