Use of Genetic Suppressor Elements to Identify Potential Breast Tumor Suppressor Genes
Abstract
To isolate new breast tumor suppressor genes GSE library specific for normal breast cells was screened for transforming elements. In parallel, ING1 candidate tumor suppressor gene, previously isolated through the screening of the same GSE library, was analyzed. Ing1 was found to be highly evolutionarily conserved gene with complex regulation, which involves generation of alternative transcripts initiated from different promoters and translated into two proteins that differ in size and expression patterns. These proteins have opposite effects on p53-regulated transcription, indicating that ing1 encodes two products with the properties of a putative tumor suppressor and a putative oncogene. Ing1 product that works as a p53 antagonist was shown to be highly expressed in different tumor cell lines and primary mouse skin tumors, which might reflect its connection with cancer development. Mice with the heterozygous deletion of ing1 gene are generated. Analysis of ing1 knockout animals should give us important insight into its possible role in cancer development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA384064
Entities
People
- Marija Zeremski
Organizations
- University of Illinois at Chicago