Molecular Mechanisms of Metastasis Suppression in Human Breast Cancer
Abstract
Our preliminary data indicated the presence of a breast carcinoma metastasis suppressor genes on human chromosome 11. The goal was to identify and begin characterizing the gene(s) responsible. Two approaches were proposed - (1) introduction of smaller pieces of chromosome 11 into cells with assessment of metastatic potential; and (2) identification of differentially expressed mRNA in metastasis suppressed cells. Progress using Approach #1 was not successful. Approach #2 was successful. We identified three novel cDNAs using differential display which were more highly expressed in the metastasis-suppressed neo11/435 hybrids. Moreover, one of those candidates, BRMS1, significantly suppressed metastasis in two human breast carcinoma cell lines when transfected and constitutively expressed. BRMS1 1 maps to 11q13, a site commonly involved in late-stage breast carcinoma. The mechanism of action appears to be novel, but was not determined during the funding period. In summary, we accomplished the stated ultimate objective for DAMD-17-96-1-6152. Results generated from DAMD-17- 96-1-6152 were used to successfully compete for funding from the National Cancer Institute to follow-up on these exciting findings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA384068
Entities
People
- Danny R. Welch
Organizations
- Pennsylvania State University