Protein Interaction with the N-Terminus of the Androgen Receptor
Abstract
The goal of this project is to identify proteins that bind to the N-terminal region of the human androgen receptor (hAR). This region is characterized by a series of CAG repeats that is variable in length in different individuals. The scope of this research is the following: 1) to identify N- terminal binding proteins using a yeast two-hybrid system, 2) to confirm the interaction of the candidate proteins with hAR in mammalian cells, 3) to investigate differential binding of these proteins to hAR with varied numbers of CAG repeats, and 4) to examine resultant levels of gene transcription by the different length hAR molecules. Our hypothesis is that unique proteins bind preferentially to hAR proteins with longer CAG repeats, negatively regulating their transcription. Bait plasmids were constructed for the yeast two-hybrid system which contain only the DNA fragment corresponding to the N-terminal 90 amino acids plus Gln repeats of two different lengths (27 and 17). Using the longer sequence as bait, a prostate cDNA library was screened and four positive clones were identified. It is our anticipation that this research will lead to new approaches to abrogate the activity of the androgen receptor in patients with prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA384350
Entities
People
- Paul H. Gumerlock
Organizations
- University of California