Microbially Mediated Reductive Dechlorination of Dichlorobenzene
Abstract
The microbially mediated reductive dechlorination of 1,2-, 1,3-, and 1 ,4-dichlorobenzene (DCB) was studied using site material from eight different locations. The soil and groundwater from each site were anaerobically added to multiple serum bottles and mixed. All three DCB isomers were added to each serum bottle to a total nominal concentration of 30 micro-mole per liter for some microcosms and 600 micro-mole per liter for other microcosms. In addition, electron donor in the form of yeast extract (100 mg/L) was added to some microcosms and some microcosms were autoclaved and served as abiotic controls. The microcosms were then incubated, inverted, in the dark at 24 deg C and periodically monitored by head space injections into a gas chromatograph system. Reductive dechlorination of the DCB isomers was noted in microcosms from five of the eight locations and ranged from 10% to nearly 100%. Additionally, in all the positive microcosms, dechlorination proceeded independent of whether yeast extract was added. Due to the rapid onset of dechlorination in some of the microcosms, it is likely that DCB dechlorination is occurring on site. Generally, 1,2-DCB was the most readily degraded DCB isomer. 1,3-DCB was the next most readily degraded isomer, distantly followed by 1,4-DCB.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA384655
Entities
People
- Anne S. Quistorff
Organizations
- Cornell University School of Civil and Environmental Engineering