Determination of Binding Constants of Water Soluble Chemicals for Biologically Based Kinetic Models
Abstract
The interaction of exogenous chemicals (xenobiotics) with endogenous biomolecules can modulate the kinetics of that chemical in biological systems. The purpose of this report is to provide a fundamental understanding of the underlying mathematical and experimental principles involved in describing these interactions. The theoretical basis of the interaction of xenobiotics with binding sites located on endogenous molecules are discussed in the context of the following scenarios: (i) interaction between a single chemical and a single class of binding sites, (il) interaction between a single chemical and multiple classes of binding sites, (iii) interactions between multiple chemicals and a single class of binding sites. Each of these situations is analyzed in the context of the reaction mechanisms involved and the mathematical description of those reactions. Experimental approaches to evaluating the basic mechanisms of binding and estimation of binding parameters using titration experiments and inhibition experiments are described. Practical instructions are provided for the conduct and interpretation of these experiments. The relationship between theoretical considerations and practical experimental consequences are highlighted.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA384696
Entities
People
- Brent Foy
- Jacoba H. Toxopeus
- John M. Frazier
- Michael Pelekis