Modulation of Adhesion Molecule Expression on Prostate Tumor Cells After Co-Culture With Eosinophilic Cell Lines
Abstract
We have demonstration that cell lines developed from metrizamide density fractions of peripheral blood hypodense (.22) and hyperdense (.24) eosinophils significantly inhibited LNCaP3, PC3, DU145 and HPC1 monolayer growth cultures in vitro. This activity was enhanced when the eosinophils were pretreated with interleukin-5. DU145 and PC3 colony formation was inhibited 50-75% by eosinophil cell lines, while peripheral blood eosinophils inhibited PC3 colony formation by as much as 95%. 24hr. cultured eosinophil supernatants significantly inhibited PC3 colony formation with 3/7 supematants causing 100% inhibition. Inhibitory activity of the 24hr. supematants towards DU145 was more variable, ranging from 9-90% inhibition of colony formation. There was slight enhancement of growth with one preparation. IL-4 and TNFalpha, which were found present in the supematants also inhibited growth of monolayers cultures of PC3, DU145 and HPC1. These data strongly support the hypothesis that eosinophils can destroy prostate tumor cells in vitro. The eosinophil cell lines and cytokine modulation of their activity offer an exquisite tool for more detailed study (both cellular and molecular) of a role for eosinophils as anti-prostate cancer effector cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA384854
Entities
People
- Paulette Furbert-harris
Organizations
- Howard University