Protein Kinase Pathways that Regulate Neuronal Survival and Death

Abstract

Loss of post-mitotic neurons from the adult brain underlies the pathology of neurodegenerative diseases and neurotoxin exposure. Neuronal cell death occurs by two mechanisms; necrosis and apoptosis. Apoptosis is a process whereby developmental cues and environmental stimuli activate a genetic program to implement a series of steps that culminate in cell death. An important aspect of apoptosis is that it can be halted and such interventions may rescue dying neurons. The overall goal of this project is to identify key protein kinases involved in regulating neuronal survival and apoptosis. The aims for the first year of funding, as described in the Statement of Work, were to: (1) Define key protein kinase cascades regulated by neutrophic factors in neurons, and (2) Modulate the protein kinase cascades regulated by neurotrophic factors and determine the consequence on neuronal survival and death. In the last year, we have identified 3 different protein kinases that regulate neuronal survival and a few of the downstream targets of these kinases. We have also shown that treatment of brain grafts with neurotrophic factors or inhibitors of stress-activated protein kinases increase survival of transplanted dopaminergic neurons in hemi-parkinsonian rats. These studies have resulted in 6 published manuscripts and 4 abstracts presented at national scientific meetings.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA384856

Entities

People

  • Kim A. Heidenreich

Organizations

  • University of Colorado Health

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Health Services
  • Neurons
  • Peptide Growth Factors

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Neuroscience

Technology Areas

  • Biotechnology