In Vitro Cytotoxicity Assays of Human Epidermal Keratinocytes in Culture Exposed to Sulfur Mustard
Abstract
A number of studies have described several in vitro cytotoxicity assays that are convenient methods for assessing cell viability. Viability assessment of human peripheral blood lymphocytes (PBL) by flow cytometry of propidium iodide (PI) exclusion has been a useful tool for screening candidate prophylactic (PI) as well as therapeutic (T) drugs for medical countermeasures against sulfur mustard (HD)) for many years at our institute. Our laboratory has reported problems associated with simple vital dye uptake as a measure of cytotoxicity in human epidermal keratinocytes (HEK) following in vitro exposure to HD. We have evaluated five alternative assays of cell viability for HEK exposed to HI) in vitro: Calcein-AM, a fluorescent dye; Alamar Blue, a redox fluorescent dye; Neutral Red, a chromogenic dye; Methyl tetrazolium salt (MTS), a colorimetric assay; and viable cell number. At the highest HI) dose tested, the viable cell number, Calcein-AM, Alamar Blue and Neutral Red assays were more sensitive than propidium iodide uptake.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2000
- Accession Number
- ADA385183
Entities
People
- Clark L. Gross
- Juantia J. Guzman
- Susan A. Kelly
- William J. Smith
Organizations
- United States Army Medical Research Institute of Chemical Defense