Determination of ATP Levels in Sulfur Mustard-Exposed Human Peripheral Blood Lymphocytes by a Chemiluminescent Assay

Abstract

Sulfur mustard (HD) is a powerful blistering agent whose mechanism is poorly understood. One of the effects of this chemical warfare agent is to lower NAD+ levels through the activation of poly (ADP-ribose) polymerase; HD also depletes ATP, the major energy supply for biochemical reactions within the cell. ATP is a biological marker that can be measured in HD-exposed cells by using a chemiluminescent reaction based on the luciferin-luciferase reaction. This assay is conveniently measured by the use of a luminometer and exhibits a reproducible linear standard curve. Three prototypic inhibitors of the poly (ADP-ribose) polymerase have been studied to determine their effect on restoring or maintaining ATP levels in HD- exposed human peripheral blood lymphocytes (PBL).

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2000
Accession Number
ADA385186

Entities

People

  • Clark L. Gross
  • Marian R. Nelson
  • Susan A. Kelly
  • William J. Smith

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Army Training
  • Biological Markers
  • Biological Sciences
  • Biomedical Research
  • Blood
  • Cells
  • Chemical Warfare
  • Chemical Warfare Agents
  • Chemical Weapons
  • Data Analysis
  • Inhibitors
  • Lymphocytes
  • Medical Personnel
  • Monitoring
  • Standards
  • Warfare

Fields of Study

  • Biology
  • Chemistry

Readers

  • Cardiovascular Physiology
  • Geochemistry
  • Molecular Photonics/Laser Physics