Novel Peptide/Protein Delivery System Targeting erbB2-Overexpressing Breast Cancer Cells
Abstract
A major goal of this Idea proposal is to determine whether the penetratin-based peptide delivery system may be developed to effectively deliver anti-cancer therapeutic peptides/proteins into breast cancer cells. We have fulfilled the objective 1 (Tasks 1-3) during the first year of the grant support. Although we experienced initial difficulties labeling the peptides with FITC, we finally were able to succeed in chemical synthesis of FITC-labeled penetratin peptides. When evaluating the translocation abilities of penetratin peptides in breast cancer cells, we were excited to find that the shortened FITC-YGRKKRRQR peptide was able to penetrate into breast cancer cells efficiently. Systematic studies of this penetratin delivery peptide in breast cancer cells, including the effective dose range, delivery efficiency, tolerable doses, stability etc. have led to better understanding of the properties of these peptides. Most importantly, we have found that the shortened FITC-YGRKKRRQR peptide can selectively translocate into the cytoplasmic subcellular compartment without going into nucleus as did the original penetratin peptide. These works pointed a very positive direction for the next grant-support year, when will test the effect of erbB2 signal-blocking peptides using penetratin delivery system, which may be developed into new therapeutic agents.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA385234
Entities
People
- Dihau Yu
Organizations
- The University of Texas MD Anderson Cancer Center