Mammary-Specific Targeting of the BRCA2 Breast Cancer Susceptibility Gene in Mice
Abstract
Women with inherited mutations in the BRCA2 gene have a very high lifetime risk of developing breast cancer. Previously, we developed a mouse with an exon 10/11 Brca2 mutation by using standard gene targeting in embryonic stem cells. Unfortunately, these mice were embryonic lethals. Therefore, we have now generated mice carrying a Cre-loxP conditional Brca2 mutated allele by flanking exon 27 with loxP sites. We predict that the site-specific recombination of loxP sites and deletion of exon 27 in this floxed Brca2 allele by a Cre recombinase protein will disrupt basic functions of Brca2 in DNA repair. The mammary-specific removal of Brca2 exon 27 by Cre-mediated recombination in vivo is performed by crossing the homozygous floxed Brca2 mice with MMTV-Cre transgenic mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1999
- Accession Number
- ADA385293
Entities
People
- Kimberely A. Mcallister
- Roger Wiseman
Organizations
- National Institute of Environmental Health Sciences