Androgen Regulation of Human Prostate Cell Growth
Abstract
The purpose of this project is to identify the androgen-regulated genes in LnCaP cells that might play a role in the regulation of prostate cancer growth. The genes were identified by gene trapping in which LnCaP cells were transfected with a selectable gene driven by a defective promoter so that only cells in which the transgene could utilize endogenous promoter elements were selected. The specific gene could then be identified by gene walking from the transgene into the surrounding endogenous DNA. At least fifty LnCaP cell clones have been identified in which androgen down-regulated promoter elements have been trapped. Two genes have been identified by gene walking thus far. One is the gene for a casein kinase previously described as expressed in the prostate and androgen regulated. The other is the gene for a peptide elongation factor EIF4A2. EIF4A2 is described as more highly expressed in skeletal muscle and prostate but has not been previously reported as androgen-regulated. These results suggest that gene trapping is a viable method of identifying hormone regulated genes and that there remain androgen-regulated genes to be discovered.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2000
- Accession Number
- ADA385309
Entities
People
- Robert W. Harrison Iii
Organizations
- University of Rochester