Cell Migration as a Therapeutic Target in Malignant Breast Cancer
Abstract
The object of this project is to develop a high-throughput method for screening potential inhibitors of breast cancer cell haptotaxis and chemotaxis, and to apply this method to identify signaling events mediating constitutive migration of malignant breast cells. The pathways that control these signaling events may be targets for development of new classes of anti-tumor drugs. The significant advances made during the first year of this project include: design and manufacture of the hardware necessary to develop the high- throughput screen, development of an efficient, high-throughput migration assay compatible with drug screening, and formation of a collaboration with the Developmental Therapeutics Program to screen their plated and natural products repositories for new inhibitors of breast cancer cell migration. Our mechanistic work has focused on construction of cell lines overexpressing normal and mutant forms of candidate signaling molecules involved in breast cell migration, including 0 protein alpha subunits, focal adhesion kinase (FAK), and crk associated substrate (CAS). Our work in the second year will focus on screening compounds and characterizing these cell lines to find new inhibitors of migration and new targets for drug design, respectively.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA385355
Entities
People
- George E. Plopper
Organizations
- University of Nevada, Las Vegas