Role of the Novel Kinase, H51, in Breast Development, Differentiation, and Carcinogenesis
Abstract
Several protein kinases involved in mammary gland development have been associated with human breast cancer and murine animal models of mammary gland carcinogenesis. To identify other protein kinases expressed in the mammary gland which may be involved in these processes, an RT-PCR degenerate oligonucleotide screen was performed on several transformed mammary epithelial cell lines and tissue derived from the mammary gland during different developmental stages. Forty-one kinases were identified, of which three were novel. One of these novel kinases, Krct, does not belong to any previously described subfamily of kinases. Analysis of the expression of murine Krct, demonstrates Krct expression in all tissues analyzed during all stages of development. Despite this wide expression pattern, Krct expression is preferentially expressed in the epithelium of several tissues including the mammary gland. Since many kinases regulate cellular proliferation and differentiation, it will be important to determine Krct's role in these processes. An analysis of the expression pattern of Krct during proliferation and differentiation has been initiated. Preliminary results demonstrate Krct may be cell cycle regulated. Since many genes that regulate the cell cycle are themselves regulated, these results suggest that Krct may regulate proliferation. Further analysis of Krct with proliferation and differentiation assays in vitro and in vivo will determine if Krct is involved in these cellular processes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA385364
Entities
People
- Douglas B. Stairs
Organizations
- University of Pennsylvania