Characterization of LAF-4, a Putative Proto-Oncogene Involved in the Development of Breast Cancer
Abstract
The long range goal of our research is to identify genes involved in the early stages of breast cancer development, specifically genes involved in the transition from hyperplasia to ductal carcinoma in-situ (DCIS) and DCIS to invasive breast cancer. We are investigating the expression of LAF-4, a gene we found to be transcriptionally perturbed in invasive breast cancer. We have selected cases of pre-malignant breast lesions and microdissected these specimens. We developed an RT-PCR assay to evaluate LAF-4 expression during progression. Quantitative RT-PCR analysis of LAF-4 niRNA level was evaluated in ANN tumor tissue in comparison to corresponding normal mammary tissue and found to be up-regulated in 2/7 cases. We have constructed riboprobes against LAF-4 and conducted several hybridisation experiments. By studying expression of LAF-4 in a spectrum of breast lesions, it may be possible to identify whether this gene plays a role in the progression to malignancy and, in the future, to develop targeted approaches to breast cancer prevention.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA385379
Entities
People
- Irene Andrulis
Organizations
- Mount Sinai Hospital, Toronto