Genetic Analysis of a Mammalian Chromosomal Origin of Replication

Abstract

The main goal of the research proposal was to develop an assay system for studying the specific genetic elements, if any, involved in the initiation of DNA replication in mammalian cells as outlined in Task 1. Since submission of the grant proposal, I have successfully developed a suitable assay for elucidating specific cis-acting elements involved in replication initiation (See attached manuscript, Altman and Fanning 2000). Briefly, a competitive polymerase chain reaction-based nascent strand abundance assay was used to demonstrate the ability of a small 5.7 kb fragment of DNA, containing the DHFR ori-beta initiation region, to support efficient origin activity when integrated into random ectopic positions in the hamster chromosome.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA385391

Entities

People

  • Amy L. Altman
  • Ellen Fanning

Organizations

  • Vanderbilt University

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biological Sciences
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Reactions
  • Chromosomes
  • Eukaryotes
  • Fungi
  • Gel Electrophoresis
  • Genetics
  • Materials
  • Molecular Biology
  • Polymerase Chain Reaction
  • Recognition
  • Two Dimensional

Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology