Mammary-Specific Gene Transfer for Modeling Breast Cancer

Abstract

Several genetic alterations have been identified in breast cancer, yet the molecular mechanisms leading to breast tumorigenesis are unknown. In order to develop a mouse model system that can dissect the genetic pathways involved in breast tumor development, we are adapting a somatic gene transfer system based on avian leukosis virus A (ALV-A) expression vectors and the TVA receptor for ALV-A. Transgenic mice have been generated that target tv-a to the mammary epithelial cells using MMTV and WAP promoters. Mammary epithelial cells from these transgenic mice are susceptible to infection both in vivo and in vitro. The animals developed should be valuable for gene delivery using the ALV-TVA vector system.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA385414

Entities

People

  • Harold E. Varmus

Organizations

  • National Institutes of Health

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cells
  • Coding
  • Diseases And Disorders
  • Epithelial Cells
  • Glands
  • Infection
  • Mammary Glands
  • Neoplasms
  • Peptide Growth Factors
  • Therapy
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech