Cloning of Novel Oncogenes Involved in Human Breast Cancer

Abstract

During the past year, we initiated our own efforts to utilize cDNA expression libraries in retrovirus vectors to screen for novel oncogenes. We have collected 8 breast cancer samples, representing either localized, noninvasive tumor tissue from invasive cancers. The rationale for choosing the noninvasive samples was that this screen would involve the identification of oncogenes involved in tumor progression, and hence, may serve as important diagnostic markers for early detection. The rationale for using the invasive tumor tissue is that these cancer cells are likely to harbor more genetic defects that noninvasive tumor cells and consequently, our chances of detection are increased. A second rationale for studying late stage cancers is that oncogenes involved promoting invasion and metastases may be identified. The identification of such genes are obviously important as both diagnostic markers for metastases and as targets for drug discovery.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2000
Accession Number
ADA385419

Entities

People

  • Channing Der

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Bioassay
  • Breast Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Detection
  • Epithelial Cells
  • Fibroblasts
  • Genetic Structures
  • Identification
  • Laboratory Animals
  • Materials
  • Neoplasms
  • North Carolina
  • Recombinant Dna

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Team-Based Human-Centered Cognitive Task Decision Making and Information Performance.

Technology Areas

  • Biotechnology