Role of STAT-3 in ER- Breast Tumors
Abstract
The hypothesis to be tested in this application is whether c-myb and A-myb genes regulate ductal cell proliferation and whether abnormalities in the expression of these genes results in breast cancer. During the first year of this study, we constructed targeting vectors that can be used for the deletion of exons 6 and 9 of mouse A-myb and c-myb genes. These vectors were transfected into embryonic stem cells and G418 resistant clones were screened for homologous recombination. Following selection, the cell lines were subjected to transient Cre expression and selection for ganciclovir-resistant clones. The ES cell clones that have undergone homologous recombination with our targeting vectors were injected into blastocysts to generate chimeric mice. To validate the results obtained from the mouse models in the human system, we have conducted parallel studies with human breast tumor cell lines. Analysis of human breast tumor cell lines showed that c-myb is expressed in a majority of ER+ human breast carcinomas, while A-myb expression is seen predominantly in ER- cell lines. Our results also shows that blockage of the biochemical function of c-myb results in a complete block to ER+ breast tumor cell proliferation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA385453
Entities
People
- Premkumar Reddy
Organizations
- Temple University