Cell Cycle Regulation of Estrogen and Androgen Receptor
Abstract
We seek to identify the phases in the cell cycle during which steroid-activated estrogen and androgen receptors are normally transcriptionally active and to determine whether this cell cycle regulation of receptor activity is maintained when cancer-inducing non- steroidal agents activate the receptors. Our hypothesis is that the activities of steroid-induced ER and AR are controlled by cell cycle regulators and that cancer-inducing, non-steroidal activators bypass or alter this regulation of receptor activity giving rise to aberrant ER and AR function. Through these studies we are gaining understanding of how estrogen and androgen receptors may contribute to the onset of tumors and to the development of hormone independent growth. We have found that the activity of the androgen and estrogen receptors is indeed regulated throughout the cell cycle with no activity of the androgen receptor in the G2/Phase phase and low activity of the estrogen receptor in cells which are mainly in S phase. Furthermore, our data indicate that non-steroidal activators of the receptors may act in a cell type and promoter dependent manner as we do not detect receptor activation by growth factors in our system.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA385514
Entities
People
- Elisabeth D. MartÃnez
Organizations
- Georgetown University