Mammary Specific Expression of Cre Recombinase Under the Control of an Endogenous MMTV LTR: A Conditional Knock-Out System
Abstract
The proposed project was to test the expression of Cre recombinase directed by the Mtv-17 locus is in its second of three years of funding. The hypothesis of the project was to develop a novel breast cancer model using the tissue-specific expression of the Mtv-17 locus, which was previously shown by this lab to only be expressed in the mammary gland. We proposed knocking-in the Cre recombinase gene at the Mtv-17 env region, so that this enzyme would only be expressed in the mammary gland. Mice that carried the Mtv-17 Cre fusion vector would then be mated to mice that had the p53 locus flanked by loxP sites, resulting in the tissue-specific loss of p53 in the mammary gland only. The prediction is that these animals would only develop mammary and not other tumors. We believed that the cre-transgenic mice would also prove useful for other investigators in the mammary gland development and tumorigenesis field.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2000
- Accession Number
- ADA385587
Entities
People
- Jennifer Czarneski
Organizations
- University of Pennsylvania