Long Interspersed Nuclear Element (LINE) Mediated Genomic Instability

Abstract

We have isolated and characterized a novel human homeobox gene designated Mix. 1 homeobox (Xenopus laevis)-like gene 2 (MIXL2), as a gene that contains a repetitive element in its 3 UTR (MER22). The MIXL2 gene encodes a predicted 232 amino acid open reading frame, which contains a highly conserved mix. 1-like homeobox and an acidic motif Radiation hybrid mapping and Fluorescence in situ hybridization localize the MIXL2 gene to human chromosome 1q32-41, a region of gain in breast tumors. Consistent with these cytogenetic findings we detected allelic imbalance for loci flanking MIXL2 in 14/25 primary breast tumors. Additionally, a search of the human dbEST database suggested a highly restricted expression as we detected only three EST clones (one each from normal germinal center B cell, undifferentiated germ cell tumor, and infiltrating ductal carcinoma of the breast). These findings suggest MT)(L2 may play a role in development and mammary carcinogenesis.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA385641

Entities

People

  • Lalitha Nagarajan

Organizations

  • The University of Texas MD Anderson Cancer Center

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Breast Cancer
  • Carcinoma
  • Cells
  • Cellular Structures
  • Chromosomes
  • Databases
  • Embryos
  • Genetic Code
  • Genetic Structures
  • Genomic Instability
  • Germ Cells
  • Human Genome
  • Instability
  • Lymphatic System
  • Neoplasms
  • Polymerase Chain Reaction

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Theoretical Analysis.