Involvement of a Novel Rho GTPase Activating Protein in Breast Tumorigenesis
Abstract
Tumor suppressors and oncogenes are central players in signal transduction pathways. Ras oncogene is one of the key intermediates that facilitates signal from upstream tyrosine kinases to a downstream cascade of serine/threonine kinases. The members of Rho family of Ras-related proteins are critical downstream components of signaling pathways. The Rho GTPases are involved in a wide spectrum of cellular functions. One of the regulators of Rho GTPases is Rho GTPase activating protein (GAP), which converts active Rho into inactive Rho. We have cloned a Rho GAP encoded by human chromosome 13q12. The sequence motifs of this Rho GAP were characterized and its expression profile in normal breast cell lines and breast carcinoma cell lines was determined. Its expression was much lower in carcinoma cell lines as compared to normal cell lines, and was undetectable in one of the carcinoma cell line tested. The Rho GAP message was highly sensitive to growth conditions. Thus mutations in Rho GAP, its reduced expression, and sensitivity to growth factors could likely enhance the levels of active Rho during breast tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA385646
Entities
People
- Rajendra Kandpal
Organizations
- Temple University