Functional Detection of p53 Mutations in Archival Prostate Cancer Tissue
Abstract
The subject of this research is 'Functional Detection of p53 Mutations in Archival Prostate Cancer Tissue'. The first aim of this research is to develop a yeast functional assay for the detection of individual p53 mutations in paraffin-embedded archival prostate cancer (CaP) tissues. The second aim is to determine the functional status of individual p53 mutations found in CaP. Although IHC and SSCP are commonly used to detect p53 mutations, their frequency is often overestimated by the former and underestimated by the latter techniques. In the first year, we have completed the evaluation of the application of this method. This includes optimizing the conditions for PCR and yeast transformation, measuring the background levels of the yeast functional assay of genomic p53 DNA, and comparing the yeast functional assay to the SSCP analysis of exon 5 of the p53 gene. Because the function of mutant p53 genes are so important to tumorigenesis and progression of cancer, these results will help in understanding the biological function of individual p53 mutations in CaP.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA385674
Entities
People
- Christopher M Evans
Organizations
- University of California