Early Detection Based on Angiogenic Growth Factors in Nipple Aspirate Fluid
Abstract
The objective of this proposal is to develop new methods of early breast cancer detection by identifying increases in angiogenic growth factor secretion in nipple aspirate fluid (NAF). Specifically, the study is examining FGF-2 (basic fibroblast growth factor) and VEGF (vascular endothelial growth factor), two of the most potent angiogenic molecules whose expression is thought to increase as an early event in breast carcinogenesis. By comparing levels of these growth factors in NAF samples from 40 women in each of three groups, i.e.; those with normal breasts, DCIS (ductal carcinoma in situ), and early invasive breast cancer, we will determine whether increases in either of these molecules heralds the transition to the preinvasive and/or invasive phenotype. In this first year of the study efforts have focused on methods and infrastructure. We have determined the most appropriate means of handling and processing the samples to prevent degradation of FGF-2 and VEGF. Accrual of patients has been slower than expected, primarily due to staffing changes at the three clinics providing subjects for this study, and a greater proportion of patients than found in our previous experience whose concern for their health status precluded interest in participating in the study. To increase accrual we have increased the percentage effort of the Research Assistant (using institutional funds) and have also engaged the efforts of additional surgeons to refer patients to the study.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA385719
Entities
People
- Bruce J Trock
Organizations
- Georgetown University