Role of ERa Splicing Variants in Regulation of AP-1 Directed Gene Transcription in Breast Cancer Cells

Abstract

This report presents recently published results from biochemical analysis of estrogen receptor (ER) -alpha mRNA splicing variants (Molecular Endocrinology 14: 634-649, May 2000). Although most of the variants appear to be functionally impaired, analysis of two receptor isoforms demonstrates that they retain many of the activities of wild-type ER-alpha, including protein-protein interaction, DNA binding, ligand binding and nuclear localization. These results and additional unpublished results from reporter gene studies suggest that ER-alpha variants can positively regulate gene transcription by a non-classical mechanism. None of the variants examined activate a consensus estrogen response element (ERE). However, specific ER-alpha variants activate the promoters of some genes that are estrogen regulated even though they lack an ERE.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2000
Accession Number
ADA385732

Entities

People

  • Aliccia Bollig
  • Richard J. Miksicek

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Anti-Bacterial Agents
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Culture Techniques
  • Epithelial Cells
  • Estrogens
  • Gene Expression
  • Growth Factors
  • Hormones
  • Materials
  • Neoplasms
  • Protein-Protein Interactions
  • Proteins
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Molecular and genetic basis of cancer.