Cell Cycle Regulation by TGFb Signaling in C. elegans
Abstract
One of the most potent inhibitors of epithelial cell growth is TGFbeta. Understanding the connection between TGFbeta and cell cycle control is an important avenue of experimentation towards treating breast cancers. We are utilizing two complementary approaches in C. elegans to find cell cycle regulatory genes that respond to TGFbeta signaling. First, in genetic screens using a cell cycle reporter gene, ribonucleotide reductase, we are looking for mutations in genes that release dauer animals (a TGFbeta induced developmental stage) from their cell cycle arrest. Secondly, we are using DNA microarrays probed with RNA from arrested animals and from animals released by TGFbeta to identify genes that are transcribed differentially. To carry out these experiments, we have constructed a new reporter gene for use in our genetic screen, and have worked out conditions to synchronize and harvest sufficient quantities of animals for making mRNA for the microarray experiments. During the next year, we will be identifying genes that are candidates for regulatory control by TGFbeta, which will offer insights into how TGFbeta control this aspect of the cell cycle.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2000
- Accession Number
- ADA385751
Entities
People
- Richard Padgett
Organizations
- Rutgers University–New Brunswick