Molecular Genetic Approaches to Biomolecular Materials

Abstract

The leukocidins are bicomponent pore-forming toxins that are structurally related to staphylococcal alpha-hemolysin, a single component toxin. alpha-Hemolysin has many applications in the area of biomolecular materials. Therefore, leukocidins have now been characterized to expand the set of tools available for materials engineering. The approach taken in the AASERT project was to obtain genes for the two components of the toxin, to express them in E. coli, to characterize the heterologously expressed pores by bilayer recording, and to use molecular genetic technology to explore structure- function relationships in these proteins. The first three of the four objectives have been accomplished.

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Document Details

Document Type
Technical Report
Publication Date
Nov 15, 2000
Accession Number
ADA385752

Entities

People

  • Hagan Bayley

Organizations

  • Texas A&M University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biochemistry
  • Biomedical And Dental Materials
  • Chemical Engineering
  • Chemistry
  • Electrical Properties
  • Engineering
  • Materials
  • Materials Engineering
  • Membrane Lipids
  • Membrane Proteins
  • Military Research
  • Protein Engineering
  • Proteins
  • Specialty Uses Of Chemicals
  • Staphylococcus Aureus

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology