Mitosis-Specific Negative Regulation of EGF-Receptor in Breast Cancer: Molecular Mechanisms, Biological Significance and Therapeutic Application
Abstract
This project is to study the M-phase specific regulation of EGF receptor (EGFR) in breast epithelial cells and to study how EGFR overexpressed cells can escape this regulation. In addition, we plan to develop a therapeutic strategy to specifically target at the EGFR overexpressors by the combination of EGF-Psuedomonas-Exotoxin (EGF-PE) with Taxol. So far, We have observed the specific phosphorylation spots of EGFR in M phase. We are currently purifying the spots and try to identify those serine/threonine phosphorylation sites and to generate the EGFR mutant with those sites mutated. The identification and mutation of those M-phase specific sites of EGFR will be very helpful for us to understand how the activity of this receptor is cell-cycle regulated. In addition, we have completed the first step in generating the tetracycline- inducible cell line for our future studies. Finally, we have basically completed our aim 3a. We found that EGF-PE could specifically target at EGFR overexpressed cells when nocodazole or taxol was combined to enrich the M-phase population of cells. The combination of EGF-PE with the M-phase specific chemodrug, such as taxol may be a potential new therapeutic approach to treat the breast cancer patients with EGFR-overexpressing tumors in the future.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA385783
Entities
People
- Shiaw-Yih Lin
Organizations
- The University of Texas MD Anderson Cancer Center