HET is a Novel Tumor Suppressor Gene in Human Breast Cancer
Abstract
We have evidence that the nuclear matrix protein HET might represent an important tumor suppressor gene in human breast cancer: 1. Overexpression of HET inhibited growth. 2. It was negatively associated with S-phase fraction in breast tumors, and 16% of breast tumors did not express HET. 3. Western Blot analysis in breast tumors led to the detection of smaller products, presumably representing truncated HET proteins. 4. HET maps to a locus on chromosome l9pl3 where we detected an unusually high rate of loss of heterozygozity. In the first specific aim we will directly answer whether HET is the tumor suppressor gene by performing additional LOB analysis and mutational analysis of BET in breast cancer cell lines as well as in tumors. In the second specific aim we will perform functional analysis of discovered BET mutations in breast cancer cell lines. If BET is the tumor suppressor gene at l9pl3, this will have a direct impact on our mechanistic understanding of tumor suppressor genes in breast cancer, as this region has an extremely high LOH rate in human breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA385800
Entities
People
- Steffi Oesterreich
Organizations
- University of Texas Health Science Center at San Antonio