Breast Cancer Associated Estrogen Receptors: Catechol Estrogen Receptors in ER-Minus Mice
Abstract
Our research will lead to a better understanding of the developmental, physiological, and biochemical roles of endogenous and environmental estrogens in breast cancer causation, prognosis and treatment. We have found in ER-alpha minus mice a uterine lactoferrin mRNA response to the catechol estrogen, 4-hydroxyestradiol, but not to estradiol. We hypothesize that the putative 4-hydroxyestradiol receptor will elicit additional responses distinct from estradiol and the classic ER-alpha and ER-beta proteins. Using this mouse model system we propose to characterize the 4-hydroxyestradiol response and the putative 4-hydroxyestradiol receptor. Specifically, we propose to: Aim #1 Characterize lactoferrin mRNA responses to 4-hydroxyestradiol in ER-minus mice; Aim #2 Characterize the putative 4-hydroxyestradiol receptor in uteri from ER-minus mice; Aim #3 Compare the specificity of the responses to 4-hydroxyestradiol with those of estradiol, 2-hydroxyestradiol, 4-methoxyestradiol, methoxychior, and tamoxifen in ER-minus mice; and Aim #4 Clone the putative 4-hydroxyestradiol receptor, which is different from both ER-alpha and ER-beta. This new 'estrogen' receptor is of major importance because of its potential involvement in novel pathways of estrogen responsiveness that may better explain estrogens' roles in breast tumor progression, prognosis, and therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA385828
Entities
People
- Dennis B. Lubahn
Organizations
- University of Missouri