New Gene Based Probes for Imaging Breast Cancer with PET
Abstract
We are developing methods to image her-2-neu oncogene over-expression in breast cancer using positron emission tomography (PET) . Small oligodeoxynucleotides (ODNs) that are complementary to the Her-2-neu messenger RNA (mRNA) are being investigated as potential imaging probes. Fluorine-l8 (2 hour half-life positron emitter) has been used to label 15-18 mer ODN probes. Furthermore, several breast and ovarian cancer cell lines which over-express ODNs have been used to study trapping of 1SF- ODNs in cell culture. We have also preliminary studied the biodistribution of ODNs in 2 living nude mice using micropET in order to understand the limitations of imaging in vivo with the antisense approach. Currently the yield of radiolabeled ODNs is very low (<20 uci), and therefore more work will have to be performed to optimize yields of the ODNs prior to proceeding further. Once ODN yields can be brought into the 150-300 uCi range, then more cell culture and in vivo experiments will be able to be performed to optimize an anti sense based approach for imaging breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA385898
Entities
People
- Senjiv S. Gambhir
Organizations
- University of California, Los Angeles