Study the Pathogenic Role of erbB-3, erbB-4 and their Ligand Heregulin in Human Breast Cancer Cells

Abstract

Among the growth factor receptors, members of the class I receptor tyrosine kinase family (ErbB) is most frequently implicated in human breast cancers. In the last report, we delineate the biological function of ErbB-4 by down-regulation of ErbB-4 expression in breast cancer cell lines with specific ErbB-4 ribozymes. To further elucidate the significance of ErbB-4 in breast cancer, we investigated the expression of ErbB-4 in primary breast carcinoma by utilizing immunohistochemical analysis with an anti-ErbB-4 monoclonal antibody on a breast cancer tissue microarray. ErbB-4 expression was found in 60% (344 of 571) of the 571 samples examined. High intense immunoreactivity of ErbB-4 was detected in 13% (74 of 571) of these primary breast tumors. Most of the staining was found in both cell membrane and cytoplasm. No expression was detected in majority of benign tumors.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2000
Accession Number
ADA386518

Entities

People

  • Careen Tang

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemistry
  • Gene Expression
  • Growth Factors
  • Materials
  • Molecules
  • Neoplasms
  • Proteins
  • Regulations
  • Tissues

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.