Response of Breast Cancer Cells to Hormonal Therapy; Quantitative in Vivo NMR Studies

Abstract

Our research is directed at determining the specific metabolic pathways and the individual reactions affected by estrogen and tamoxifen in both estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) breast cancer cells through the use of novel NMR methodology and quantitative analysis. In this award period, we have quantified the effect of estrogen on metabolic fluxes in ER+ cells. This was accomplished using a comprehensive metabolic model and NMR data from cell extracts. The model was further refined to allow for the incorporation of isotopomer, or multiply-labeled, metabolite data. Microcarrier culture, flask, and roller bottle studies were continued in order to expand the methodology used to quantify breast cancer cell metabolism under varied treatment conditions.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2000
Accession Number
ADA386522

Entities

People

  • Douglas S Clark

Organizations

  • University of California, Berkeley

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkenes
  • Basic Programming Language
  • Biodegradation
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Engineering
  • Chemistry
  • Computational Science
  • Culture Techniques
  • Cytological Techniques
  • Engineering
  • Fungi
  • Mass Spectrometry
  • Metabolism
  • Neoplasms
  • Tissue Extracts

Readers

  • Breast cancer cell signaling and growth regulation.
  • Coastal Oceanography