Role of cdc37 in Breast Cancer
Abstract
Cdc37 was initially discovered as a cell cycle gene in the yeast Saccharomyces cerevisiae. Its mammalian counterpart interacts with growth regulating kinases including CDK4, c-src, and Raft 1. Our work has shown that most of cdc37 is in a constitutive complex with the chaperone protein HSP9O. We further have found that cdc37 targets HSP90 to Rafti and is required for Raf activation. In order to understand the potential role of cdc37 in breast cancer, we have evaluated the effect of the HSP9O inhibitor geldanamycin of the growth of the MCF-7 cell line. We have found that this drug is a potent growth inhibitor of this cell line. We are currently examining the effects of antisense cdc37 and dominant negative cdc37 on the growth of this and other breast cancer cells. Although cdc37 has been reported to not be expressed in non%al murine breast tissue, we have found one human breast cancer cell line that has an amplification of the cdc37 genetic locus and has increased expression of the cdc37 protein. These data suggest that cdc37 plays an important role in the growth of some human breast cancers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA386623
Entities
People
- Brent H. Cochran
Organizations
- Tufts University