16 K Prolactin as an Angiogenic Inhibitor in Breast Cancer
Abstract
Tumors must induce the formation of new blood vessels to grow and metastasize. Prolactin (PRL) is a 23 kDa hormone that has mitogenic, morphogenic, and lactogenic actions on the breast, but its role in breast cancer is unclear. Its N-terminal 16K fragment suppresses endothelial cell proliferation in vifro, but its ability to inhibit tumor growth has not been tested. Highly specific antibodies against hPRL were generated and used to develop a novel dot blot/chemiluminescence assay to rapidly measure PRL. Baculovirus expression vectors encoding 16K or 23K hPRL were transfected into insect cells, and PRL secretion verified by Western blotting. Partially purified recombinant 16K hPRL inl%bited proliferation of bovine aortic endothelial cells in a dose-dependent manner, while 23K hPRL had no effect. %% human breast cancer cell line MDA-MB-435 expresses the angiogenic factors vEGF and FGF-2 but not PRL or its receptor. These cells develop tumors and metastases when injected into nude mice. Stable MDA-MB-435 cells that over- express 16K or 23K hPRL have been generated and will be used to determine if 16K hPRL suppresses the growth of breast cancer and metastases in nude mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2000
- Accession Number
- ADA386634
Entities
People
- Karen Liby
- Nira Ben-jonathan
Organizations
- University of Cincinnati