Expression of Transforming Growth Factor-Beta (TGF-B) in Prostate Cancer Progression
Abstract
The objective of the project was to evaluate the role of TGF-Beta in prostate cancer progression. During the past finding period, we have made significant progress. We investigated if a non-immunogenic Dunning's rat prostate cancer cell line, MATLyLu, can become immunogenic by reducing the endogenous production of TGF-Beta1. An expression construct containing a DNA sequence in an antisense orientation to TGF-Beta1 (TGF-Beta1 antisense) was stably transfected into MATLyLu cells. Following transfection, cellular content of TGF-Beta1 reduced from 70 pg to 10 pg per 2x104 cells and the rate of in vitro 3H-thymidine incorporation increased 3-5 fold. After subcutaneous injection of tumor cells into syngeneic male hosts (Copenhagen rats), the tumor incidence was 100% (15/15) for the wild type MATLyLu cells and cells transfected with the control construct, but only 43% (9/21, p < 0.05) for cells transfected with TGF-Beta1 antisense. However, when cells were injected into immunodeficient hosts (athymic nude rats), the incidence of tumor development was 100% (10/10) for both the wild type MATLyLu cells and cells transfected with the control construct and 90% (9/10) for cells transfected with TGF-Beta1 antisense. These observations support the concept that MATLyLu cells are immunogenic, when the endogenous production of TGF-Beta1 is down regulated. The above work has been accepted for publication in the British Journal of Cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2000
- Accession Number
- ADA386635
Entities
People
- Lee Chung
Organizations
- Northwestern University