Use of Genetic Engineering to Produce a Mutated Cytochrome P450 Enzyme Capable of Both Oxidizing and Reductively Dechlorinating Hazardous Organic Chemicals

Abstract

The goal of this project is to develop new catalysts and organisms that will be capable of enhancing in situ bioremediation. Our approach is to prepare site-specific mutants of the P450 monooxygenase enzyme P450 102 (BM-3) from the soil bacterium Bacillus megaterium, that possess the ability to catalyze transformations of recalcitrant organic compounds that are hazardous environmental contaminants. In this investigation we focused our effort on designing, producing. Purifying, and studying site-specific mutant of the wild type (nonmutated) P450 102 that can catalyze the oxidation of polycyclic aromatic compounds (PAHs) such as benzoApyrene and mutants that can catalyze the reductive dechlorination of organochlorine compounds such as pentachloroethane.

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Document Details

Document Type
Technical Report
Publication Date
Nov 17, 2000
Accession Number
ADA386808

Entities

People

  • David A. Mullin
  • William L. Alworth

Organizations

  • Tulane University of Louisiana

Tags

DTIC Thesaurus Topics

  • Aromatic Compounds
  • Aromatic Polycyclic Hydrocarbons
  • Biology
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Cytochromes
  • Engineering
  • Environmental Pollutants
  • Genetic Engineering
  • Genetically Modified Organisms
  • Military Research
  • Molecular Biology
  • Monitoring
  • Organic Compounds
  • Oxidation
  • Scientists

Fields of Study

  • Environmental science

Readers

  • Analytical Chemistry
  • Groundwater Contamination Remediation.
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation