Chromatin HMG-I(Y) as a Co-Regulatory Protein for Estrogen Receptor Action in Breast Cancer Cells

Abstract

As sequence specific transcription factors, progesterone (PR) and estrogen receptor (ER) mediate the actions of progesterone and estrogen through direct activation of target genes. There is increasing evidence that PR and ER can also mediate non-genomic effects through activation of the Src/Ras/Erk signaling pathway. Using PR-A as the target in a yeast two hybrid screen, the SH3 domain of c-Cbl-associated protein (CAP) was identified to interact with the N-terminal domain of PR in a hormone dependent manner in vivo. PR was found to interact directly in vitro with the SH3 domain of CAP as well as selected SH3 domains of other signaling molecules including: c-Src and Hck. The N-domain of PR contains a sequence that conforms to a class II polyproline helix (PPII) ligand for SH3 domains and was shown by mutagenesis to be required for PR interaction with SH3 domains.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2000
Accession Number
ADA386810

Entities

People

  • Viroj Boonyaratanakornkit

Organizations

  • University of Colorado Health

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical And Dental Materials
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Displacement
  • Estrogens
  • Hormones
  • Mammary Glands
  • Materials
  • Molecules
  • Neoplasms
  • Progesterone
  • Proteins
  • Tyrosine

Fields of Study

  • Biology
  • Chemistry

Readers

  • Aerial Delivery - Logistics and Supply Chain Management.
  • Breast cancer cell signaling and growth regulation.