Regulatory Pathways Involved in Heregulin-Induced Proliferation and Differentiation of Human Breast Cancer Cells
Abstract
The polypeptide heregulin has been shown to reverse the transformed phenotype of certain cultured breast cancer cell lines and hence convert them to more normal, milk-producing cells. In contrast, heregulin is reported to induce proliferation of other breast cancer cell lines. These diverse effects of heregulin are mediated by receptors in the Erb/HER family, which function as coreceptor complexes. How the different signaling pathways activated by ErbB family receptors can elicit alternatively the differentiation or proliferation of breast cancer cells was investigated in the previous year of funding. Our experiments addressed the mechanism of activation of mitogen-activated protein kinase (MAPK) by hergulin coreceptors. As one read-out of MAPK activation, we monitored the nuclear translocation of the activated enzyme by confocal immunofluorescence microscopy.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2000
- Accession Number
- ADA386811
Entities
People
- Huaming Tan
- John Koland
Organizations
- University of Iowa