Role of Estrogen Receptor Target Genes in Breast Cancer
Abstract
Prolonged exposure of estrogen is closely correlated with the increased incidence of breast cancer. To study the role of estrogen receptor (ER) target genes in the development of breast cancer we construct the regulable repressor KEDPK to directly shutdown the expression of ER target genes. The KEDPK shows a dose-dependent inhibition of ER target genes in transient transfection as says in the presence of its ligand RU486. Stable cell lines expressing KEDPK have been established in both estrogen-dependent (MCF-7) and -independent (LTSD) breast cancer cell lines. Expression of KEDPK inhibits the growth of estrogen-independent cell growth, with no effect on the cell proliferation of estrogen-dependent MCF-7 cells. KEDPK shows 5 to 10% inhibitory effect on the expression of endogenous ER target gene p52. Currently we have devoted our effort to achieve a better repression of endogenous ER target genes in breast cancer cell lines. The success of developing these repressors will allow us to study the role of ER target genes in breast cancer progression. Results obtained in these studies will be highly relevant to efforts in optimizating the current hormone therapy and gene therapy for breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2000
- Accession Number
- ADA386880
Entities
People
- Sophia Y. Tsai
- Zhi-qing Ma
Organizations
- Baylor College of Medicine