Breast Cancer Prevention by a Soybean protein
Abstract
The goal of this project is to develop Bowman-Birk protease inhibitor (BBI), a soybean polypeptide, as a chemopreventive agent for breast cancer. In order to achieve this goal, we proposed the following two specific aims in our original application: (1) using an in vitro mammary gland culture system to demonstrate the anti-transformation activity of both BBI and its palmitic acid conjugate (Pal-BBI), and (2) using a mouse model to demonstrate the advantages of Pal-BBI in oral delivery of BBI. During the funding period of this project, we have obtained 4 important results. First, we developed a new method to conjugate fatty acid to BBI via the intramolecular disulfide bonds in the polypeptide. Second, we demonstrated that BBI can prevent the transformation of mammary glands induced by the treatment of a chemical carcinogen, 7,12- dimethylbenAanthracene (DMBA). Third, we demonstrated that Pal-BBI is a better chemopreventive agent than BBI in mammary gland transformation assay. Finally, we demonstrated that Pal-BBI is more stable than the native BBI in mouse gastrointestinal tract. However, we failed to demonstrate that Pal-BBI could significantly improve the oral bioavailability in mouse model. Results from our studies will be useful for developing effective diets or chemopreventive agents for the prevention of breast cancer in the future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA386894
Entities
People
- Wei C. Shen
Organizations
- University of Southern California