Fluid Flow Sensitivity of Bone Cells as a Function of Age
Abstract
In the second year of the project we have completed aim one and have completed half of aim two. For aim one we have demonstrated our hypothesis to be true, namely that gap junctional intercellular communication modulates the PGE2 response of bone cells to oscillating fluid flow. Interestingly, we found that PGE2 release in response to oscillating flow does not appear to involve intracellular calcium. This has important implications because it indicates that other second messengers, such as the cyclic AMP pathway, may be important in transducing the fluid flow signal into the PGE2 response. Additionally, the differential effect of oscillating fluid flow on intracellular calcium versus PGE2 may prove to be a powerful tool in further investigations of the bone cell mechanotransduction pathway. We have begun work on our second task (aim 2) and have determined that while the Ca(sup 2)+i response to fluid flow does decrease as a function of age, gap junctional intercellular communication does not. These results have produced numerous scientific presentations and abstracts as well as one manuscript in review and two manuscripts in preparation. Finally, the results have been incorporated into two applications to the NIH which have been approved for funding.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2000
- Accession Number
- ADA387129
Entities
People
- Christopher R. Jacobs
Organizations
- Penn State Milton S. Hershey Medical Center